AustralieFrV1, Main, Exploration, bibRecord, 000C91

Estrogen-like and tissue-selective effects of 7-methoxycoumarin from Ficus umbellata (Moraceae): an in vitro and in vivo study

Identifieur interne : 000C91 ( Main/Exploration ); précédent : 000C90; suivant : 000C92

Estrogen-like and tissue-selective effects of 7-methoxycoumarin from Ficus umbellata (Moraceae): an in vitro and in vivo study

Auteurs : Stéphane Zingue [Cameroun, Afrique du Sud] ; Thomas Michel [France] ; Chantal Beatrice Magne Nde ; Amstrong Nang Njuh [Cameroun] ; Julia Cisilotto ; Derek Tantoh Ndinteh [Afrique du Sud] ; Colin Clyne ; Xavier Fernandez [France] ; Tânia Beatriz Creczynski-Pasa ; Dieudonné Njamen [Afrique du Sud, Cameroun]

Source :

BMC Complementary and Alternative Medicine [ 1472-6882 ] ; 2017.

RBID : PMC:5541738

Descripteurs français

KwdFr :
- Animaux, Antagonistes des oestrogènes (pharmacologie), Cellules HEK293, Cellules MCF-7, Extraits de plantes (pharmacologie), Femelle, Ficus (), Humains, Inhibiteurs de l'aromatase (pharmacologie), Lipides (sang), Oestradiol (métabolisme), Oestradiol (pharmacologie), Oestrogènes (métabolisme), Ombelliférones (pharmacologie), Os et tissu osseux (), Ovariectomie, Phyto-oestrogènes (pharmacologie), Post-ménopause, Rat Wistar, Tissu adipeux (métabolisme), Utérus (), Utérus (métabolisme), Vagin (), Vagin (métabolisme), Épithélium ().
MESH :
- métabolisme : Oestradiol, Oestrogènes, Tissu adipeux, Utérus, Vagin.
- pharmacologie : Antagonistes des oestrogènes, Extraits de plantes, Inhibiteurs de l'aromatase, Oestradiol, Ombelliférones, Phyto-oestrogènes.
- sang : Lipides.
- Animaux, Cellules HEK293, Cellules MCF-7, Femelle, Ficus, Humains, Os et tissu osseux, Ovariectomie, Post-ménopause, Rat Wistar, Utérus, Vagin, Épithélium.

English descriptors

KwdEn :
- Adipose Tissue (metabolism), Animals, Aromatase Inhibitors (pharmacology), Bone and Bones (drug effects), Epithelium (drug effects), Estradiol (metabolism), Estradiol (pharmacology), Estrogen Antagonists (pharmacology), Estrogens (metabolism), Female, Ficus (chemistry), HEK293 Cells, Humans, Lipids (blood), MCF-7 Cells, Ovariectomy, Phytoestrogens (pharmacology), Plant Extracts (pharmacology), Postmenopause, Rats, Wistar, Umbelliferones (pharmacology), Uterus (drug effects), Uterus (metabolism), Vagina (drug effects), Vagina (metabolism).
MESH :
- chemical , blood : Lipids.
- chemical , metabolism : Estradiol, Estrogens.
- chemical , pharmacology : Aromatase Inhibitors, Estradiol, Estrogen Antagonists, Phytoestrogens, Plant Extracts, Umbelliferones.
- chemistry : Ficus.
- drug effects : Bone and Bones, Epithelium, Uterus, Vagina.
- metabolism : Adipose Tissue, Uterus, Vagina.
- Animals, Female, HEK293 Cells, Humans, MCF-7 Cells, Ovariectomy, Postmenopause, Rats, Wistar.

Abstract

Background

Ficus umbellata is a medicinal plant previously shown to endow estrogenic properties. Its major component was isolated and characterized as 7-methoxycoumarin (MC). Noteworthy, coumarins and the respective active metabolite 7-hydroxycoumarin analogs have shown aromatase inhibitory activity, which is of particular interest in the treatment of estrogen-dependent cancers. The present work aimed at evaluating the estrogenic/antiestrogenic effects of MC in vitro and in vivo.

Methods

To do so, in vitro assays using E-screen and reporter gene were done. In vivo, a 3-day uterotrophic assay followed by a postmenopausal-like rat model to characterize MC as well as F. umbellata aqueous extract in ovariectomized Wistar rats was performed. The investigations focused on histological (vaginal and uterine epithelial height) and morphological (uterine wet weight, vagina stratification and cornification) endpoints, bone mass, biochemical parameters and lipid profile.

Results

MC induced a significant (p < 0.05) MCF-7 cell proliferation at a concentration of 0.1 μM, but did not inhibit the effect induced by estradiol in both E-screen and reporter gene assays. In vivo, MC treatment did not show an uterotrophic effect in both rat models used. However, MC (1 mg/kg) induced a significant increase (p < 0.01) of vaginal epithelial height. No significant change was observed with MC in abdominal fat weight, serum lipid levels and bone weight.

Conclusion

These results suggest that MC has a weak estrogenic activity in vitro and in vivo that accounts only in part to the estrogenicity of the whole plant extract. MC could be beneficial with regard to vagina dryness as it showed a tissue specific effect without exposing the uterus to a potential tumorigenic growth.

Electronic supplementary material

The online version of this article (doi:10.1186/s12906-017-1895-9) contains supplementary material, which is available to authorized users.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541738

DOI: 10.1186/s12906-017-1895-9
PubMed: 28768532
PubMed Central: 5541738

Affiliations:

Afrique du Sud, Cameroun, France

Le document en format XML

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<author><name sortKey="Zingue, Stephane" sort="Zingue, Stephane" uniqKey="Zingue S" first="Stéphane" last="Zingue">Stéphane Zingue</name>
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<author><name sortKey="Njamen, Dieudonne" sort="Njamen, Dieudonne" uniqKey="Njamen D" first="Dieudonné" last="Njamen">Dieudonné Njamen</name>
<affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0109 131X</institution-id>
<institution-id institution-id-type="GRID">grid.412988.e</institution-id>
<institution>Department of Applied Chemistry, Faculty of Science,</institution>
<institution>University of Johannesburg,</institution>
</institution-wrap>
Doornfontein, 2028 South Africa</nlm:aff>
<country xml:lang="fr">Afrique du Sud</country>
<wicri:regionArea>Doornfontein</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="Aff6"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2173 8504</institution-id>
<institution-id institution-id-type="GRID">grid.412661.6</institution-id>
<institution>Department of Animal Biology and Physiology, Faculty of Science,</institution>
<institution>University of Yaoundé 1,</institution>
</institution-wrap>
P.O. Box 812, Yaounde, Cameroon</nlm:aff>
<country xml:lang="fr">Cameroun</country>
<wicri:regionArea>P.O. Box 812, Yaounde</wicri:regionArea>
<wicri:noRegion>Yaounde</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">BMC Complementary and Alternative Medicine</title>
<idno type="eISSN">1472-6882</idno>
<imprint><date when="2017">2017</date>
</imprint>
</series>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adipose Tissue (metabolism)</term>
<term>Animals</term>
<term>Aromatase Inhibitors (pharmacology)</term>
<term>Bone and Bones (drug effects)</term>
<term>Epithelium (drug effects)</term>
<term>Estradiol (metabolism)</term>
<term>Estradiol (pharmacology)</term>
<term>Estrogen Antagonists (pharmacology)</term>
<term>Estrogens (metabolism)</term>
<term>Female</term>
<term>Ficus (chemistry)</term>
<term>HEK293 Cells</term>
<term>Humans</term>
<term>Lipids (blood)</term>
<term>MCF-7 Cells</term>
<term>Ovariectomy</term>
<term>Phytoestrogens (pharmacology)</term>
<term>Plant Extracts (pharmacology)</term>
<term>Postmenopause</term>
<term>Rats, Wistar</term>
<term>Umbelliferones (pharmacology)</term>
<term>Uterus (drug effects)</term>
<term>Uterus (metabolism)</term>
<term>Vagina (drug effects)</term>
<term>Vagina (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Antagonistes des oestrogènes (pharmacologie)</term>
<term>Cellules HEK293</term>
<term>Cellules MCF-7</term>
<term>Extraits de plantes (pharmacologie)</term>
<term>Femelle</term>
<term>Ficus ()</term>
<term>Humains</term>
<term>Inhibiteurs de l'aromatase (pharmacologie)</term>
<term>Lipides (sang)</term>
<term>Oestradiol (métabolisme)</term>
<term>Oestradiol (pharmacologie)</term>
<term>Oestrogènes (métabolisme)</term>
<term>Ombelliférones (pharmacologie)</term>
<term>Os et tissu osseux ()</term>
<term>Ovariectomie</term>
<term>Phyto-oestrogènes (pharmacologie)</term>
<term>Post-ménopause</term>
<term>Rat Wistar</term>
<term>Tissu adipeux (métabolisme)</term>
<term>Utérus ()</term>
<term>Utérus (métabolisme)</term>
<term>Vagin ()</term>
<term>Vagin (métabolisme)</term>
<term>Épithélium ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Lipids</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Estradiol</term>
<term>Estrogens</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Aromatase Inhibitors</term>
<term>Estradiol</term>
<term>Estrogen Antagonists</term>
<term>Phytoestrogens</term>
<term>Plant Extracts</term>
<term>Umbelliferones</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Ficus</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Bone and Bones</term>
<term>Epithelium</term>
<term>Uterus</term>
<term>Vagina</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Adipose Tissue</term>
<term>Uterus</term>
<term>Vagina</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Oestradiol</term>
<term>Oestrogènes</term>
<term>Tissu adipeux</term>
<term>Utérus</term>
<term>Vagin</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antagonistes des oestrogènes</term>
<term>Extraits de plantes</term>
<term>Inhibiteurs de l'aromatase</term>
<term>Oestradiol</term>
<term>Ombelliférones</term>
<term>Phyto-oestrogènes</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Lipides</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Female</term>
<term>HEK293 Cells</term>
<term>Humans</term>
<term>MCF-7 Cells</term>
<term>Ovariectomy</term>
<term>Postmenopause</term>
<term>Rats, Wistar</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules HEK293</term>
<term>Cellules MCF-7</term>
<term>Femelle</term>
<term>Ficus</term>
<term>Humains</term>
<term>Os et tissu osseux</term>
<term>Ovariectomie</term>
<term>Post-ménopause</term>
<term>Rat Wistar</term>
<term>Utérus</term>
<term>Vagin</term>
<term>Épithélium</term>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p id="Par1"><italic>Ficus umbellata</italic>
 is a medicinal plant previously shown to endow estrogenic properties. Its major component was isolated and characterized as 7-methoxycoumarin (MC). Noteworthy, coumarins and the respective active metabolite 7-hydroxycoumarin analogs have shown aromatase inhibitory activity, which is of particular interest in the treatment of estrogen-dependent cancers. The present work aimed at evaluating the estrogenic/antiestrogenic effects of MC in vitro and in vivo.</p>
</sec>
<sec><title>Methods</title>
<p id="Par2">To do so, in vitro assays using E-screen and reporter gene were done. In vivo<italic>,</italic>
 a 3-day uterotrophic assay followed by a postmenopausal-like rat model to characterize MC as well as <italic>F. umbellata</italic>
 aqueous extract in ovariectomized Wistar rats was performed. The investigations focused on histological (vaginal and uterine epithelial height) and morphological (uterine wet weight, vagina stratification and cornification) endpoints, bone mass, biochemical parameters and lipid profile.</p>
</sec>
<sec><title>Results</title>
<p id="Par3">MC induced a significant (<italic>p</italic>
 < 0.05) MCF-7 cell proliferation at a concentration of 0.1 μM, but did not inhibit the effect induced by estradiol in both E-screen and reporter gene assays. In vivo, MC treatment did not show an uterotrophic effect in both rat models used. However, MC (1 mg/kg) induced a significant increase (<italic>p</italic>
 < 0.01) of vaginal epithelial height. No significant change was observed with MC in abdominal fat weight, serum lipid levels and bone weight.</p>
</sec>
<sec><title>Conclusion</title>
<p id="Par4">These results suggest that MC has a weak estrogenic activity in vitro and in vivo that accounts only in part to the estrogenicity of the whole plant extract. MC could be beneficial with regard to vagina dryness as it showed a tissue specific effect without exposing the uterus to a potential tumorigenic growth.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s12906-017-1895-9) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Davison, Sl" uniqKey="Davison S">SL Davison</name>
</author>
<author><name sortKey="Bell, R" uniqKey="Bell R">R Bell</name>
</author>
<author><name sortKey="Donath, S" uniqKey="Donath S">S Donath</name>
</author>
<author><name sortKey="Montalto, Jg" uniqKey="Montalto J">JG Montalto</name>
</author>
<author><name sortKey="Davis, Sr" uniqKey="Davis S">SR Davis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Thomas, F" uniqKey="Thomas F">F Thomas</name>
</author>
<author><name sortKey="Renaud, F" uniqKey="Renaud F">F Renaud</name>
</author>
<author><name sortKey="Bebefice, E" uniqKey="Bebefice E">E Bebefice</name>
</author>
<author><name sortKey="De Meeus, T" uniqKey="De Meeus T">T De Meeus</name>
</author>
<author><name sortKey="Guegan, Jf" uniqKey="Guegan J">JF Guegan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nkwo, P" uniqKey="Nkwo P">P Nkwo</name>
</author>
<author><name sortKey="Onah, H" uniqKey="Onah H">H Onah</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mc Clung, Mr" uniqKey="Mc Clung M">MR Mc Clung</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yang, Xp" uniqKey="Yang X">XP Yang</name>
</author>
<author><name sortKey="Reckelhoff, Jf" uniqKey="Reckelhoff J">JF Reckelhoff</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bhavnani, Br" uniqKey="Bhavnani B">BR Bhavnani</name>
</author>
<author><name sortKey="Strickler, Rc" uniqKey="Strickler R">RC Strickler</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rossouw, Je" uniqKey="Rossouw J">JE Rossouw</name>
</author>
<author><name sortKey="Anderson, Gl" uniqKey="Anderson G">GL Anderson</name>
</author>
<author><name sortKey="Prentice, Rl" uniqKey="Prentice R">RL Prentice</name>
</author>
<author><name sortKey="Lacroix, Az" uniqKey="Lacroix A">AZ LaCroix</name>
</author>
<author><name sortKey="Kooperberg, C" uniqKey="Kooperberg C">C Kooperberg</name>
</author>
<author><name sortKey="Stefanick, Ml" uniqKey="Stefanick M">ML Stefanick</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Skouby, So" uniqKey="Skouby S">SO Skouby</name>
</author>
<author><name sortKey="Al Azzawi, F" uniqKey="Al Azzawi F">F Al-azzawi</name>
</author>
<author><name sortKey="Barlow, D" uniqKey="Barlow D">D Barlow</name>
</author>
<author><name sortKey="Calaf Aldina Erdogan Ertungel, Pj" uniqKey="Calaf Aldina Erdogan Ertungel P">PJ Calaf-aldina erdogan ertüngel</name>
</author>
<author><name sortKey="Gompel, A" uniqKey="Gompel A">A Gompel</name>
</author>
<author><name sortKey="Graziottin, A" uniqKey="Graziottin A">A Graziottin</name>
</author>
<author><name sortKey="Hudita, D" uniqKey="Hudita D">D Hudita</name>
</author>
<author><name sortKey="Pines, A" uniqKey="Pines A">A Pines</name>
</author>
<author><name sortKey="Rozenberg, S" uniqKey="Rozenberg S">S Rozenberg</name>
</author>
<author><name sortKey="Samsioe, G" uniqKey="Samsioe G">G Samsioe</name>
</author>
<author><name sortKey="Stevenson, Jc" uniqKey="Stevenson J">JC Stevenson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cheema, D" uniqKey="Cheema D">D Cheema</name>
</author>
<author><name sortKey="Coomarasamy, A" uniqKey="Coomarasamy A">A Coomarasamy</name>
</author>
<author><name sortKey="El Toukhy, T" uniqKey="El Toukhy T">T El-toukhy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Boue, Sm" uniqKey="Boue S">SM Boué</name>
</author>
<author><name sortKey="Tilghman, Sl" uniqKey="Tilghman S">SL Tilghman</name>
</author>
<author><name sortKey="Elliott, S" uniqKey="Elliott S">S Elliott</name>
</author>
<author><name sortKey="Zimmerman, Mc" uniqKey="Zimmerman M">MC Zimmerman</name>
</author>
<author><name sortKey="Williams, Ky" uniqKey="Williams K">KY Williams</name>
</author>
<author><name sortKey="Payton Stewart, F" uniqKey="Payton Stewart F">F Payton-Stewart</name>
</author>
<author><name sortKey="Miraflor, Ap" uniqKey="Miraflor A">AP Miraflor</name>
</author>
<author><name sortKey="Howell, Mh" uniqKey="Howell M">MH Howell</name>
</author>
<author><name sortKey="Shih, By" uniqKey="Shih B">BY Shih</name>
</author>
<author><name sortKey="Carter Wientjes, Ch" uniqKey="Carter Wientjes C">CH Carter-Wientjes</name>
</author>
<author><name sortKey="Segar, C" uniqKey="Segar C">C Segar</name>
</author>
<author><name sortKey="Beckman, Bs" uniqKey="Beckman B">BS Beckman</name>
</author>
<author><name sortKey="Wiese, Te" uniqKey="Wiese T">TE Wiese</name>
</author>
<author><name sortKey="Cleveland, Te" uniqKey="Cleveland T">TE Cleveland</name>
</author>
<author><name sortKey="Mclachlan, Ja" uniqKey="Mclachlan J">JA McLachlan</name>
</author>
<author><name sortKey="Burow, Me" uniqKey="Burow M">ME Burow</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Zingue, S" uniqKey="Zingue S">S Zingue</name>
</author>
<author><name sortKey="Michel, T" uniqKey="Michel T">T Michel</name>
</author>
<author><name sortKey="Tchatchou, J" uniqKey="Tchatchou J">J Tchatchou</name>
</author>
<author><name sortKey="Magne Nde, Cb" uniqKey="Magne Nde C">CB Magne Nde</name>
</author>
<author><name sortKey="Winter, E" uniqKey="Winter E">E Winter</name>
</author>
<author><name sortKey="Monchot, A" uniqKey="Monchot A">A Monchot</name>
</author>
<author><name sortKey="Awounfack, Cf" uniqKey="Awounfack C">CF Awounfack</name>
</author>
<author><name sortKey="Djiogue, S" uniqKey="Djiogue S">S Djiogue</name>
</author>
<author><name sortKey="Clyne, C" uniqKey="Clyne C">C Clyne</name>
</author>
<author><name sortKey="Fernandez, X" uniqKey="Fernandez X">X Fernandez</name>
</author>
<author><name sortKey="Creczynski Pasa, Tb" uniqKey="Creczynski Pasa T">TB Creczynski-Pasa</name>
</author>
<author><name sortKey="Njamen, D" uniqKey="Njamen D">D Njamen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="De Souza, Lg" uniqKey="De Souza L">LG De Souza</name>
</author>
<author><name sortKey="Renn, Mn" uniqKey="Renn M">MN Renn</name>
</author>
<author><name sortKey="Figueroa Villar, Jd" uniqKey="Figueroa Villar J">JD Figueroa-Villar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jacquot, Y" uniqKey="Jacquot Y">Y Jacquot</name>
</author>
<author><name sortKey="Laios, I" uniqKey="Laios I">I Laïos</name>
</author>
<author><name sortKey="Cleeren, A" uniqKey="Cleeren A">A Cleeren</name>
</author>
<author><name sortKey="Nonclercq, D" uniqKey="Nonclercq D">D Nonclercq</name>
</author>
<author><name sortKey="Bermont, L" uniqKey="Bermont L">L Bermont</name>
</author>
<author><name sortKey="Refouvelet, B" uniqKey="Refouvelet B">B Refouvelet</name>
</author>
<author><name sortKey="Boubekeur, K" uniqKey="Boubekeur K">K Boubekeur</name>
</author>
<author><name sortKey="Xicluna, A" uniqKey="Xicluna A">A Xicluna</name>
</author>
<author><name sortKey="Leclercq, G" uniqKey="Leclercq G">G Leclercq</name>
</author>
<author><name sortKey="Laurent, G" uniqKey="Laurent G">G Laurent</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Musa, Ma" uniqKey="Musa M">MA Musa</name>
</author>
<author><name sortKey="Cooperwood, Js" uniqKey="Cooperwood J">JS Cooperwood</name>
</author>
<author><name sortKey="Khan, Mof" uniqKey="Khan M">MOF Khan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Resende, Fa" uniqKey="Resende F">FA Resende</name>
</author>
<author><name sortKey="De Oliveira, Aps" uniqKey="De Oliveira A">APS De Oliveira</name>
</author>
<author><name sortKey="De Camargo, Ms" uniqKey="De Camargo M">MS De Camargo</name>
</author>
<author><name sortKey="Vilegas, W" uniqKey="Vilegas W">W Vilegas</name>
</author>
<author><name sortKey="Varanda, Ea" uniqKey="Varanda E">EA Varanda</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schiliro, T" uniqKey="Schiliro T">T Schiliro</name>
</author>
<author><name sortKey="Gorrasi, I" uniqKey="Gorrasi I">I Gorrasi</name>
</author>
<author><name sortKey="Longo, A" uniqKey="Longo A">A Longo</name>
</author>
<author><name sortKey="Coluccia, S" uniqKey="Coluccia S">S Coluccia</name>
</author>
<author><name sortKey="Gilli, G" uniqKey="Gilli G">G Gilli</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zingue, S" uniqKey="Zingue S">S Zingue</name>
</author>
<author><name sortKey="Magne Nde, Cb" uniqKey="Magne Nde C">CB Magne Nde</name>
</author>
<author><name sortKey="Clyne, C" uniqKey="Clyne C">C Clyne</name>
</author>
<author><name sortKey="Njamen, D" uniqKey="Njamen D">D Njamen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zingue, S" uniqKey="Zingue S">S Zingue</name>
</author>
<author><name sortKey="Tchoumtchoua, J" uniqKey="Tchoumtchoua J">J Tchoumtchoua</name>
</author>
<author><name sortKey="Ntsa, Dm" uniqKey="Ntsa D">DM Ntsa</name>
</author>
<author><name sortKey="Sandjo, Lp" uniqKey="Sandjo L">LP Sandjo</name>
</author>
<author><name sortKey="Cisilotto, J" uniqKey="Cisilotto J">J Cisilotto</name>
</author>
<author><name sortKey="Magne Nde, Cb" uniqKey="Magne Nde C">CB Magne Nde</name>
</author>
<author><name sortKey="Winter, E" uniqKey="Winter E">E Winter</name>
</author>
<author><name sortKey="Awounfack, Cf" uniqKey="Awounfack C">CF Awounfack</name>
</author>
<author><name sortKey="Ndinteh, Dt" uniqKey="Ndinteh D">DT Ndinteh</name>
</author>
<author><name sortKey="Clyne, C" uniqKey="Clyne C">C Clyne</name>
</author>
<author><name sortKey="Njamen, D" uniqKey="Njamen D">D Njamen</name>
</author>
<author><name sortKey="Halabalaki, M" uniqKey="Halabalaki M">M Halabalaki</name>
</author>
<author><name sortKey="Creczynski Pasa, Tb" uniqKey="Creczynski Pasa T">TB Creczynski-Pasa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dzeufiet, Dpd" uniqKey="Dzeufiet D">DPD Dzeufiet</name>
</author>
<author><name sortKey="Mengue, Nys" uniqKey="Mengue N">NYS Mengue</name>
</author>
<author><name sortKey="Bilanda, Dc" uniqKey="Bilanda D">DC Bilanda</name>
</author>
<author><name sortKey="Kameni, M" uniqKey="Kameni M">M Kameni</name>
</author>
<author><name sortKey="Aboubakar, Ob F" uniqKey="Aboubakar O">OB-F Aboubakar</name>
</author>
<author><name sortKey="Dimo, T" uniqKey="Dimo T">T Dimo</name>
</author>
<author><name sortKey="Kamtchouing, P" uniqKey="Kamtchouing P">P Kamtchouing</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ammar, S" uniqKey="Ammar S">S Ammar</name>
</author>
<author><name sortKey="Contreras, Mm" uniqKey="Contreras M">MM Contreras</name>
</author>
<author><name sortKey="Belguith Hadrich, O" uniqKey="Belguith Hadrich O">O Belguith-Hadrich</name>
</author>
<author><name sortKey="Bouaziz, M" uniqKey="Bouaziz M">M Bouaziz</name>
</author>
<author><name sortKey="Segura Carretero, A" uniqKey="Segura Carretero A">A Segura-Carretero</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Jimenez Orozco, Fa" uniqKey="Jimenez Orozco F">FA Jiménez-Orozco</name>
</author>
<author><name sortKey="Rosales, Aar" uniqKey="Rosales A">AAR Rosales</name>
</author>
<author><name sortKey="Vega L Pez, A" uniqKey="Vega L Pez A">A Vega-López</name>
</author>
<author><name sortKey="Dominguez L Pez, Ml" uniqKey="Dominguez L Pez M">ML Domínguez-López</name>
</author>
<author><name sortKey="Garcia Mondrag N, Mj" uniqKey="Garcia Mondrag N M">MJ García-Mondragón</name>
</author>
<author><name sortKey="Maldonado Espinoza, A" uniqKey="Maldonado Espinoza A">A Maldonado-Espinoza</name>
</author>
<author><name sortKey="Lemini, C" uniqKey="Lemini C">C Lemini</name>
</author>
<author><name sortKey="Mendoza Pati O, N" uniqKey="Mendoza Pati O N">N Mendoza-Patiño</name>
</author>
<author><name sortKey="Mandoki, Jj" uniqKey="Mandoki J">JJ Mandoki</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kuiper, Gg" uniqKey="Kuiper G">GG Kuiper</name>
</author>
<author><name sortKey="Carlsson, B" uniqKey="Carlsson B">B Carlsson</name>
</author>
<author><name sortKey="Grandien, K" uniqKey="Grandien K">K Grandien</name>
</author>
<author><name sortKey="Enmark, E" uniqKey="Enmark E">E Enmark</name>
</author>
<author><name sortKey="Haggblad, J" uniqKey="Haggblad J">J Haggblad</name>
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<author><name sortKey="Chaichantipyuth, C" uniqKey="Chaichantipyuth C">C Chaichantipyuth</name>
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<author><name sortKey="Ishikawa, T" uniqKey="Ishikawa T">T Ishikawa</name>
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   |texte=   Estrogen-like and tissue-selective effects of 7-methoxycoumarin from Ficus umbellata (Moraceae): an in vitro and in vivo study
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Serveur d'exploration sur les relations entre la France et l'Australie

Estrogen-like and tissue-selective effects of 7-methoxycoumarin from Ficus umbellata (Moraceae): an in vitro and in vivo study

Estrogen-like and tissue-selective effects of 7-methoxycoumarin from Ficus umbellata (Moraceae): an in vitro and in vivo study

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	Serveur d'exploration sur les relations entre la France et l'Australie
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